An upper limit for macromolecular crowding effects

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چکیده

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An upper limit for macromolecular crowding effects

BACKGROUND Solutions containing high macromolecule concentrations are predicted to affect a number of protein properties compared to those properties in dilute solution. In cells, these macromolecular crowders have a large range of sizes and can occupy 30% or more of the available volume. We chose to study the stability and ps-ns internal dynamics of a globular protein whose radius is ~2 nm whe...

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Macromolecular crowding: obvious but underappreciated.

Biological macromolecules evolve and function within intracellular environments that are crowded with other macromolecules. Crowding results in surprisingly large quantitative effects on both the rates and the equilibria of interactions involving macromolecules, but such interactions are commonly studied outside the cell in uncrowded buffers. The addition of high concentrations of natural and s...

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Effects of macromolecular crowding on the collapse of biopolymers.

Experiments show that macromolecular crowding modestly reduces the size of intrinsically disordered proteins even at a volume fraction (ϕ) similar to that in the cytosol, whereas DNA undergoes a coil-to-globule transition at very small ϕ. We show using a combination of scaling arguments and simulations that the polymer size R̅(g)(ϕ) depends on x=R̅(g)(0)/D, where D is the ϕ-dependent distance bet...

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ژورنال

عنوان ژورنال: BMC Biophysics

سال: 2011

ISSN: 2046-1682

DOI: 10.1186/2046-1682-4-13